FRET-Based Optical Assay for Monitoring Riboswitch Activation
نویسندگان
چکیده
منابع مشابه
FRET-based optical assay for monitoring riboswitch activation.
Riboswitches are regulatory RNAs located in the 5'-untranslated region of mRNA sequences that recognize and bind to small molecules and regulate the expression of downstream genes. Creation of synthetic riboswitches to novel ligands depends on the ability to monitor riboswitch activation in the presence of analyte. In our work, we have coupled a synthetic riboswitch to an optical reporter assay...
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Riboswitches are a type of natural genetic control element that use untranslated sequence in the RNA to recognize and bind to small molecules that regulate expression of that gene. Creation of synthetic riboswitches to novel ligands depends on the ability to screen for analyte binding sensitivity and specificity. In our work, we have coupled a synthetic riboswitch to an optical reporter assay b...
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ژورنال
عنوان ژورنال: Biomacromolecules
سال: 2009
ISSN: 1525-7797,1526-4602
DOI: 10.1021/bm801117f